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α casp 1  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology α casp 1
    α Casp 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+casp+1/SC514/pm40953119-228-98-99
    Average 94 stars, based on 319 article reviews
    α casp 1 - by Bioz Stars, 2026-09
    94/100 stars

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    gene-expression assays: human il-1β, tnf-α, casp-1, asc nlrp3  (Thermo Fisher)
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    Characterization of PBMCs from CRMO patients. (A-E) Real Time-PCR analysis of gene products involved in the regulation <t>of</t> <t>IL-1β,</t> including <t>ASC</t> , NLRP3 , CASP-1 , IL-1β and of the pro-inflammatory cytokine TNF-α in freshly isolated PBMCs obtained from CRMO patients in remission (gray dots) or with active disease (black dots) and healthy controls (white dots). Values are presented as arbitrary unit (AU). (F-G) IL-1β released in supernatants by PBMCs isolated from CRMO patients in remission or in active disease and from healthy controls, after stimulation with 10 ng/ml LPS for 3 hours (F) or 10 ng/ml LPS for 2 hours followed by treatment with 2 mM ATP for 1 hour (G) . IL-1β was measured by ELISA. Black lines represent the mean value. *p < 0.05, **p < 0.01 vs healthy controls.
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    Characterization of PBMCs from CRMO patients. (A-E) Real Time-PCR analysis of gene products involved in the regulation of IL-1β, including ASC , NLRP3 , CASP-1 , IL-1β and of the pro-inflammatory cytokine TNF-α in freshly isolated PBMCs obtained from CRMO patients in remission (gray dots) or with active disease (black dots) and healthy controls (white dots). Values are presented as arbitrary unit (AU). (F-G) IL-1β released in supernatants by PBMCs isolated from CRMO patients in remission or in active disease and from healthy controls, after stimulation with 10 ng/ml LPS for 3 hours (F) or 10 ng/ml LPS for 2 hours followed by treatment with 2 mM ATP for 1 hour (G) . IL-1β was measured by ELISA. Black lines represent the mean value. *p < 0.05, **p < 0.01 vs healthy controls.

    Journal: Pediatric Rheumatology Online Journal

    Article Title: Deregulation of the IL-1β axis in chronic recurrent multifocal osteomyelitis

    doi: 10.1186/1546-0096-12-30

    Figure Lengend Snippet: Characterization of PBMCs from CRMO patients. (A-E) Real Time-PCR analysis of gene products involved in the regulation of IL-1β, including ASC , NLRP3 , CASP-1 , IL-1β and of the pro-inflammatory cytokine TNF-α in freshly isolated PBMCs obtained from CRMO patients in remission (gray dots) or with active disease (black dots) and healthy controls (white dots). Values are presented as arbitrary unit (AU). (F-G) IL-1β released in supernatants by PBMCs isolated from CRMO patients in remission or in active disease and from healthy controls, after stimulation with 10 ng/ml LPS for 3 hours (F) or 10 ng/ml LPS for 2 hours followed by treatment with 2 mM ATP for 1 hour (G) . IL-1β was measured by ELISA. Black lines represent the mean value. *p < 0.05, **p < 0.01 vs healthy controls.

    Article Snippet: Real-time PCR assays were performed using TaqMan Universal PCR Master mix (Applied Biosystems) and the following gene-expression assays: human IL-1β, TNF-α, CASP-1, ASC and NLRP3 (Applied Biosystem).

    Techniques: Real-time Polymerase Chain Reaction, Isolation, Enzyme-linked Immunosorbent Assay